What is the chemical structure of 4- (2-Aminoethyl) Benzenesulfonyl Fluoride Hydrochloride?
4- (2-aminoethyl) benzenesulfonyl fluorohydrochloride, the chemical structure of which is related to the field of organic chemistry. This compound contains a group of benzene ring, a group of sulfonyl fluoride on one side of the benzene ring, and a chain of 2-aminoethyl on the other side.
Looking at its benzene ring, it is a six-membered carbon ring with a conjugated π electronic system. It is the core structure of the compound, giving it certain stability and special electronic effects. In the sulfonyl fluoride group, the sulfur atom is connected to the dioxygen atom by a double bond and is connected to the fluorine atom. This structure makes the sulfonyl fluoride highly reactive. Due to the characteristics of the electron cloud density distribution around the sulfur atom, it is easy to participate in nucleophilic substitution and other reactions.
As for the chain of 2-aminoethyl, it is formed by connecting the ethane group to the amino group. The amino group has a lone pair of electrons and is basic, which can participate in the protonation reaction or react with the electrophilic reagent, while the ethane group is a flexible carbon chain, which affects the spatial conformation and lipophilicity of the molecule. The form of hydrochloride salt indicates that the amino group forms a salt of hydrochloric acid, which increases the solubility of the compound in water. Because it forms an ionic structure, it can form a strong interaction In this way, the various parts of the structure cooperate with each other, resulting in the unique chemical properties and reactivity of 4 - (2-aminoethyl) benzenesulfonyl fluorohydrochloride.
What are the main uses of 4- (2-Aminoethyl) Benzenesulfonyl Fluoride Hydrochloride
4- (2 -Aminoethyl) benzenesulfonyl fluorohydrochloride is often used as a protease inhibitor and has a wide range of uses in biochemical and cellular research.
It is mainly used in protein-related experiments. During protein extraction, cells or tissues contain a variety of proteases, which will degrade the target protein and hinder research. This reagent can inhibit protease activity, maintain protein integrity, preserve its structure and function, and improve experimental accuracy and reliability. For example, when studying the function of a specific protein, the protein is extracted from tissue. Adding this reagent can prevent the protein from being decomposed by proteases during the extraction process.
In the field of enzyme activity research, the mechanism of enzymes in physiological and pathological processes can be studied by inhibiting the activity of specific proteases. Scientists add it to the reaction system to observe changes in enzyme activity and its impact on downstream pathways, explore the mechanism of disease occurrence and development, and find potential targets for drug development.
In cell culture experiments, cell metabolism secretes proteases, which affect cell growth, proliferation and differentiation. Adding this reagent can regulate the cell microenvironment, ensure normal physiological functions of cells, and provide a stable environment for cell experiments. For example, in culturing nerve cells, adding it can prevent extracellular matrix proteases from damaging nerve cells and help nerve cell growth and differentiation research.
What is the safety of 4- (2-Aminoethyl) Benzenesulfonyl Fluoride Hydrochloride?
4 - (2 -Aminoethyl) benzenesulfonyl fluorohydrochloride, this is a chemical substance. Its safety is of paramount importance and needs to be investigated in detail.
This substance is potentially harmful. First, it can cause irritation to the skin. If the skin is accidentally touched, it may be red, swollen and itchy. If it is not properly handled in time after contact, it may cause more serious discomfort. Second, it is especially irritating to the eyes. If it splashes into the eyes, it may cause severe pain, tears, and in severe cases, it may damage the vision. Third, inhalation of this substance may be unfavorable to the respiratory tract, causing cough, asthma and other diseases, and even breathing difficulties.
When handling this substance, protective measures must be comprehensive. Operators should wear protective clothing and protective gloves to avoid skin contact. At the same time, protective glasses must be worn to prevent substances from splashing into the eyes. The workplace should be well ventilated to reduce the concentration of this substance in the air and reduce the risk of inhalation.
If contact is inadvertent, emergency treatment needs to be prompt and correct. If skin contact, rinse immediately with a lot of water, and then decide whether to seek medical attention according to the specific situation. If eye contact, rinse immediately with a lot of water and seek medical attention as soon as possible. If inhaled, leave the scene quickly and go to a fresh air place. If the symptoms are serious, be sure to seek medical attention in time.
In conclusion, the safety of 4- (2-aminoethyl) benzenesulfonyl fluorohydrochloride should not be underestimated. Strict safety procedures should be followed during operation, and protection and emergency preparedness should be done to ensure personnel safety and health.
What are the storage conditions for 4- (2-Aminoethyl) Benzenesulfonyl Fluoride Hydrochloride?
4 - (2 -Aminoethyl) benzenesulfonyl fluorohydrochloride, a commonly used reagent in biochemical research, has the properties of inhibiting serine protease. Its storage conditions are quite critical, as follows:
First, temperature control is extremely important. It should be stored in a low temperature environment. Generally speaking, it needs to be placed in a frozen environment of -20 ° C. Low temperature can effectively slow down the chemical reaction rate of the reagent itself, reduce the possibility of degradation, and then maintain its chemical stability and biological activity. Due to the increase in temperature, it is easy to cause molecular movement to intensify, making the structure of the reagent more susceptible to change.
Second, moisture-proof measures are indispensable. The reagent is easy to absorb moisture, and moisture will trigger reactions such as hydrolysis, which will damage its quality Therefore, it should be stored in a dry place or in an airtight container containing desiccant to ensure that the impact of environmental humidity on it is minimized.
Third, it cannot be ignored to be stored away from light. Light or photochemical reactions affect the stability of the reagent. It can be stored in an opaque container, or stored in a dark place to avoid direct light exposure.
Fourth, avoid repeated freezing and thawing. Multiple freezing and thawing processes will change the volume of the reagent solution due to changes in the shape of water, generate pressure, destroy the structure of the reagent, and may cause local concentration changes and affect the activity. Therefore, if you need to use it, you should use it as needed to avoid unnecessary freezing and thawing cycles. When storing 4- (2-aminoethyl) benzenesulfonyl fluorohydrochloride, the principles of low temperature, drying, avoiding light, and avoiding repeated freezing and thawing can ensure its quality and performance, and play its due role in the experiment.
4- (2-Aminoethyl) Benzenesulfonyl Fluoride Hydrochloride
The synthesis method of 4 - (2 -aminoethyl) benzenesulfonyl fluorohydrochloride is of interest in the field of organic synthesis.
In the past, the synthesis of this compound followed the path of classical organic synthesis. One method is to use benzenesulfonyl chloride as the starting material and react with appropriate amine compounds. If benzenesulfonyl chloride is reacted with 2-aminoethanol under suitable reaction conditions, such as in an inert solvent, using an organic base as the acid binding agent, controlling its temperature and reaction time, the corresponding intermediate can be obtained. This intermediate is further converted, such as converting the alcohol hydroxyl group into a leaving group, then reacting with a nucleophilic reagent to introduce a fluorine atom, and then being acidified to obtain the target product 4- (2-aminoethyl) benzenesulfonyl fluorohydrochloride.
There is another method, starting with a benzene ring derivative, first introducing a sulfonyl group, through a series of functional group transformations, introducing an aminoethyl group, then introducing a fluorine atom, and finally forming a salt. For example, the benzene ring is first sulfonated to obtain a benzenesulfonic acid derivative, and the aminoethyl group is introduced through esterification, aminolysis, and then introducing a fluorine atom through a halogenation reaction, and finally reacting with hydrochloric acid to form a salt.
In the process of synthesis, the control Temperature, solvent, ratio of reactants, choice of catalyst, etc., all affect the yield and selectivity of the reaction. And the separation and purification after each step of the reaction cannot be ignored. Appropriate separation methods, such as column chromatography, recrystallization, etc., are required to ensure the purity of the intermediate and final product, so as to obtain high-quality 4- (2-aminoethyl) benzenesulfonyl fluoride hydrochloride.